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Automatic Bone Perimeter is used to automatically trace the outer and void perimeter of the bone surface. Artifical edges created where the bone touches the region of interest are automatically excluded. |
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BIOQUANT automatically traces and measures bone volume and bone surface. This is possible on a variety of stains including von Kossa Tetrachrome and Masson Trichrome.
The Automatic Bone Perimeter array is used to automatically measure bone surface. This special array automatically excludes the artificial surface created when the bone touches the edges of the Image window or Rectangle ROI. It also automatically includes the void surfaces within the bone surface. This save the user substantial time.
When Segment Assignment is used, specialty surfaces, such as Erosion Surface, can be distributed to their corresponding arrays based on the Automatic Bone Perimeter measurement tracing. Quiescient Surface is automatically calculated and does not need to be distributed. Osteoid Surface is usually distributed using the Osteoid Tool.
To distribute a specialty surface type:
1) Click the Segment Assignment button to move the cursor to the Image window.
2) Click once on the measurement tracing where the first surface type begins.
3) Click once on the measurement tracing where the first surface type ends.
4) On the keyboard, press the number that is assigned to that surface type.
Once a number is pressed, the system measures the length of the segment along the bone mass and changes the measurement tracing to its associated color.
5) The procedure is repeated for all surface types in the image.
6) The "H" key on the keyboard may be used to hide the tracings to see underlying tissue.
Segments are color coded by type. Measurements across multiple fields
are added cumulatively in the appropriate arrays.
Common Surface Type Arrays:
OS(Ob+) Osteoid: Osteoblast +*
OS(Ob-) Osteoid: Osteoblast -*
ES(Oc+) Erosion: Osteoclast +
ES(Oc-) Erosion: Osteoclast -
Artifical Surface (Crack)**
Undefined Surface
* Osteoid Surfaces are usually defined using the Osteoid Tool, instead of Segment Assignment.
** In BIOQUANT OSTEO II, the artifical edge created when the bone touches the full screen or rectangle region of interest is automatically excluded from the Total Perimeter measurement. In this case, artifical surface does not need to be defined by the user. However, if there was a crack or tear in the tissue, it would need to be distributed as Artifical Surface.