Inactivating mutations that lead to loss of heterozygosity within the HRPT2/Cdc73 gene are directly linked to the development of primary hyperparathyroidism, parathyroid adenomas, and ossifying fibromas of the jaw (HPT-JT). The protein product of the Cdc73 gene, parafibromin, is a core member of the polymerase-associated factors (PAF) complex, which coordinates epigenetic modifiers and transcriptional machinery to control gene expression. We conditionally deleted Cdc73 within mesenchymal progenitors or within mature osteoblasts and osteocytes to determine the consequences of parafibromin loss within the mesenchymal lineage.
N-cadherin Regulation of Bone Growth and Homeostasis Is Osteolineage Stage–Specific
N-cadherin inhibits osteogenic cell differentiation and canonical Wnt/β-catenin signaling in vitro. However, in vivo both conditional Cdh2 ablation and overexpression in osteoblasts lead to low bone mass. We tested the hypothesis that N-cadherin has different effects on osteolineage cells depending upon their differentiation stage. Embryonic conditional osteolineage Cdh2 deletion in mice results in defective growth, low bone mass, and reduced osteoprogenitor number.
Effectiveness of conservative interventions for sickness and pain behaviors induced by a high repetition high force upper extremity task
Systemic inflammation is known to induce sickness behaviors, including decreased social interaction and pain. We have reported increased serum inflammatory cytokines in a rat model of repetitive strain injury (rats perform an upper extremity reaching task for prolonged periods). Here, we sought to determine if sickness behaviors are induced in this model and the effectiveness of conservative treatments.
Human tooth-derived biomaterial as a graft substitute for hard tissue regeneration
The present study was conducted to evaluate the efficacy of human dentine grafts for new bone augmentation. Materials & methods: Dentine grafts (demineralized dentine matrix [DDM] and mineralized dentine matrix [MDM]) were prepared and implanted in rats. Tetracycline was administered twice. Paraffin and resin sections were prepared from the harvested grafts and stained respectively with hematoxylin and eosin (in addition to tartrate acid phosphatase for osteoclasts) and Villanueva.
Tissue Damage Assessments in Total Hip Arthoplasty
Decreasing tissue damage and recovery time, while improving quality of life have been the focus of many approaches to total hip arthroplasty (THA). In this study, we compared two approaches, a tissue-sparing superior capsulotomy percutaneously assisted approach (SP) and the traditional posterior approach (TR), to address the question of whether the novel technique reduces tissue damage. The secondary aim of this study focused on the measurement technologies utilized to quantify the damage resulting from either SP or TR. Image J, BioQuant, and cellSens were the image analysis programs employed. Statistical validation and comparisons of results between all platforms were performed.
Fkbp10 Deletion in Osteoblasts Leads to Qualitative Defects in Bone
Osteogenesis imperfecta (OI), also known as brittle bone disease, displays a spectrum of clinical severity from mild (OI type I) to severe early lethality (OI type II), with clinical features including low bone mass, fractures, and deformities. Mutations in the FK506 Binding Protein 10 (FKBP10), gene encoding the 65-kDa protein FKBP65, cause a recessive form of OI and Bruck syndrome, the latter being characterized by joint contractures in addition to low bone mass.