BIOQUANT OSTEO II

Bone histomorphometry can be done quickly and accurately. We've been developing automated measurement tools more than 25 years. It's time to put them to use in your research.

All the Help You Need

• A community of the most prestigious research labs, major pharmaceutical companies, and most trusted contract research organizations.
• Annual workshop to discuss tissue processing, bone pathology, and software procedures.
• Complimentary on-site training with new systems in the USA and Canada.
• Complimentary telephone technical support.
• Referrals to independent histomorphometry consultants available.

All the Methods You Use

• Trabecular and cortical bone in tibia frontal sections, vertebral sections, and iliac crest biopsy.
• Cortical bone assessment in femoral cross-sections.
• Tumor metastasis in trabecular bone.
• Mouse calvaria injection assay analysis.
• Orthopaedic implant assessment.

All the Control You Want

• High-precision automatic tracing tools with undo support.
• Manual tracing tools with undo support.
• All the standard ASBMR indices.
• As many custom indices as you want.
• High resolution digital imaging.

Continuous Development Provides New Features You Request

BIOQUANT is committed to keeping OSTEO II at the leading edge of analysis and imaging technology. Our software systems are in constant development, adding features in response to customer requests, adding support for new digital camera and measuring stages, and planning for new PC and operating system platforms.

Flexible Tissue Volume Referent Tools

• Define a rectangular tissue volume of any size simply by typing the necessary width and height.
• Define an irregular tissue volume of any shape with a drawing tool that ensures you are a set distance away from cortical walls and other anatomical boundaries.
• The tissue volume is defined conveniently at low magnification, but the boundary also appears enlarged during measurement at higher magnification, ensuring that measurements remain within the tissue volume referent.

Fast and Accurate Bone Volume and Bone Surface Tools

• Using BIOQUANT's unique detection algorithms bone is automatically recognized by color and size.
• Easily identified stains include: Goldner's and Masson's Trichrome, McNeil's Tetrachrome, Von Kossa, and Toluidine blue.
• Automatic tools (with undo) measure the bone volume, taking into account void spaces on the interior.
• Automatic Bone Perimeter measures total bone volume, including void surfaces, and excluding artificial surface created by the image edge.
• Surface Type Assignment lets you categorize a portion of the bone surface to erosion surface just by clicking the beginning and end of the erosion surface. The tracing of bone surface can be hidden so that the erosion surface is clearly visible.
• The Osteoid Wizard simultaneously measures osteoid volume, osteoid surface, and osteoid thickness.
• Complete test of manual tracing tools with undo for unstained sections.

Powerful Labeled Surface Tools

• Simple tracing tools let you measure the length of single labeled surface.
• The Double Labeled Surface tool simultaneously measures the length of double labeled surface and the interlabel distance.

Easy Data Export and Presentation Imaging

• Dynamic calculations box displays current values
• Data of animal groups are exported to Excel workbooks with a simple Data Export Wizard.
• 1.3 megapixel images may be instantly captured during measurement for procedural documentation and illustration of journal articles.
• Images may be stamped with calibration bars, text labels, and arrows.

Features new to OSTEO II Since ASBMR 2006

• Automatic Bone Perimeter can now be used with irregular and rectangular tissue volumes.
• Using Surface Type Assignment, you can now identify erosion and osteoid surfaces directly on the image, without the bone and tissue volume tracings covering the bone surface.
• Undo is now supported for most automatic and manual measurements.
• The Overview Window, which contains a map of measured portions of the section, now has a "You are Here" box.
• Data are now saved automatically every few minutes and every few kilobytes.

BIOQUANT Life Science

BIOQUANT Life Science provides tools specifically designed for biomedical and materials analysis. New tools increase data accuracy, speed up measurement, enhance image resolution, and simplify data export.

Selected New and Improved Features in BIOQUANT Life Science at its plug-ins

Read about these and more features in the BIOQUANT Life Science brochure.

New Area Percent Wand for Grain Labeling Analysis

1. Threshold the grains.
2. Use a circular wand to mark all the cells in the image (or manually trace around cells).
3. Click the Measure button.
4. The software:
   • Measures the grain area in each cell and the area of each cell.
   • Estimates the number of grains over the cell and calculates grain density per cell.
   • Calculates the ratio of cellular grain density to background grain density to determine positively labeled cells.
   • Saves the topography of measured cells to prevent double counting.

• Analyze sequences of image completely automatically using field mode or automatic mode.
• Load 100, 1,000, or 10,000 images at a time depending on which Imaging Toolkit (if any) is installed with the system.
• Specify threshold ranges and some threshold editing options.
• Specify the arrays to be measured and the measurement order.
• Add loops to repeat measurements over many images.

Auto Width Tool for Measuring Cortex Layer Thickness in Coronal Sections

1. Draw outer border of the layer.
2. Draw inner border.
3. The software generates width measurements between the two borders, perpendicular to the outer border.

Stereology Toolkit Plug-in Enhancements to Stereology Probes

• Optical Fractionator CE calculations per Schmitz and Hof, 2000.
• The software automatically tabulates tissue thickness from disector locations to determine mean tissue thickness.
• New Fakir probe for measuring tissue volume and tissue surface area simultaneously.
• If your system has the Imaging Toolkit, you can montage the sampling area so students can trace it on one field of view.
• See Stereology Toolkit brochure.

Stereology Toolkit Plug-in New Optical Fractionator Data Manager

• Don't worry about managing data sets. The project, its animals, and the sections measured for each animal are all handled here.
• It keeps track of how many sections you've measured for the animal and the current N and CE values for the animal.
• It's simple to create projects, add new animals to a project and new sections to an animal.
• It's easy to audit data collected earlier, and to resample a section with problems.
• Data for an entire project are exported directly to Excel. You get the N and CE values for every animal in the group and all the raw data to calculate it yourself.
• See Stereology Toolkit brochure.

New Densitometry Toolkit Plug-in

• For receptor binding assays and other quantitative densitometry applications.
• Customizable curve-fitting calibration to radiostandards showing r^2 value of fit and relative error of every standard.
• Density data are collected in calibrated units. (Automatic Mode, Field Mode, Density Wand, Manual Mode).
• Histograms display data in calibrated units.
• Create pseudocolor tables from calibration standards.
• Stamp pseudocolor scale bars on image, in calibrated units.
• Support for QIMAGING RETIGA EXI Monochrome Cameras.
• Support for FUJIFILM BAS5000 Scanner IMG files.
• See Densitometry Toolkit brochure.

Improved Imaging Toolkit Plug-in

• Use a thumbnail navigator to review and capture image sequences.
• Merge multiple fluorescent images into a single, multi-channel image.
• Merge bright field images into fluorescent images to indicated localization of labels.
• Save BIF images which encode calibration and XY position data into the image.
• Push a button to montage overlapping BIF images into a single seamless image.
• Load up to 1000 images in a sequence.
• See Imaging Toolkit brochure.

Improved Automated Imaging Toolkit

• Capture up to 10,000 images, depending on system resources.
• Control a motorized stage to digitize slides for analysis on satellite systems.
• Collect images for batch measurement and montage.
• See Automated Imaging Toolkit brochure.