Cellular mRNA Analysis
Image the slide
BIOQUANT can handle up to 4GB slide scans, or measure directly on a live microscope image. Save the measurement tracings on the scan for easy reproducible results and quality assurance.
Semi-automated Grain Counting
S35 radiolabeling and silver grain emulsion dipping identify mRNA signals in tissue sections.
Using either standardized circular regions or free-drawn boundaries, BIOQUANT can analyze the degree of grain labeling in a cell population.
Grains are automatically detected by color thresholding. BIOQUANT calculates the grain density for each cell. Cells with a grain density more than 5X the background grain density are considered significantly labeled.
DATA COLLECTED
Mean Grain Size
Estimated Background Grain Density
Estimated Background Grain Number
% Cellular Grain Area
Estimated Cellular Grain Number
Estimated Cellular Grain Density
Cellular Grain Density Ratio
MEASURE AVG AREA OF ONE GRAIN AND BACKGROUND GRAINS
Find the average size of one grain to help estimate the number of grains for total number of grains, total grains in the background, total grains on cells.
MEASURE GRAINS PER CELLS
BIOQUANT automatically estimates the number of grains overlapping cells. The % grains per cell is automatically calculated.
MOVE TO AN OVERLAPPING FIELD OF VIEW
BIOQUANT keeps track of previously measured grains and cells, providing tracings that scale with the image if you decide to measure on a different zoom level.